ChloroquineV1, Main, Exploration, bibRecord, 003571

Intracellular transport and esterification of exchangeable cholesterol in cultured human lung fibroblasts

Identifieur interne : 003571 ( Main/Exploration ); précédent : 003570; suivant : 003572

Intracellular transport and esterification of exchangeable cholesterol in cultured human lung fibroblasts

Auteurs : J. Peter Slotte [Suède] ; Bo Lundberg [Suède] ; Sören Björkerud [Finlande]

Source :

Biochimica et Biophysica Acta (BBA) - Lipids and Lipid Metabolism [ 0005-2760 ] ; 1984.

RBID : ISTEX:7F0235A400FF7773783075F40D982C51B89B428A

Descripteurs français

KwdFr :
- Cellules cultivées, Cholestérol (métabolisme), Cholestérol ester (métabolisme), Colchicine (pharmacologie), Cyanures (pharmacologie), Cytochalasine B (pharmacologie), Fluorures (pharmacologie), Humains, Phospholipides (métabolisme), Pinocytose (), Poumon (métabolisme).
MESH :
- métabolisme : Cholestérol, Cholestérol ester, Phospholipides, Poumon.
- pharmacologie : Colchicine, Cyanures, Cytochalasine B, Fluorures.
- Cellules cultivées, Humains, Pinocytose.

English descriptors

KwdEn :
- Cells, Cultured, Cholesterol (metabolism), Cholesterol Esters (metabolism), Colchicine (pharmacology), Cyanides (pharmacology), Cytochalasin B (pharmacology), Fluorides (pharmacology), Humans, Lung (metabolism), Phospholipids (metabolism), Pinocytosis (drug effects).
MESH :
- chemical , metabolism : Cholesterol, Cholesterol Esters, Phospholipids.
- chemical , pharmacology : Colchicine, Cyanides, Cytochalasin B, Fluorides.
- drug effects : Pinocytosis.
- metabolism : Lung.
Teeft :
- Biol, Cell biol, Cell membranes, Cell protein, Cells, Cultured, Cellular cholesterol, Cellular pinocytosis, Chloroquine, Cholesterol exchange, Cholesterol exchange process, Cholesterol mass determination, Cholesteryl esters, Colchicine, Cold saline, Conical centrifuge tubes, Control cells, Cultured cells, Cultured mesenchymal cells, Cultured mouse fibroblasts, Cytochalasin, Different drugs, Drugs colchicine, Energy poisons, Ester, Ester formation, Esterification, Esterification sites, Exchange process, Exchangeable, Exchangeable cholesterol, Fetal calf serum, Fibroblast, Growth medium, Human lung fibroblasts, Human skin fibroblasts, Humans, Incubation medium, Incubation period, Intracellular, Intracellular esterification sites, Intracellular fate, Intracellular transport, Lipid, Lipid vesicles, Lung fibroblasts, Lysosomal compartment, Mass transfer, Membrane flow, Metabolic energy, Metabolic inhibitors, Model system, Molecular cholesterol, Molecular exchange, Muscle cells, Pinocytosis, Plasma membranes, Results show, Surface transfer, Total cholesterol, Tracer, Tracer uptake, Unesterified cholesterol, Unpublished data, Unspecific binding, Vesicle.

Abstract

Abstract: We have studied the intracellular fate of exchangeable cholesterol in a model system with lipid vesicles (cholesterol/phospholipid mole ratio 1:1) and cultured human lung fibroblasts. Exchangeable [3H]cholesterol in lipid vesicles was readily incorporated into cellular plasma membranes and transported to intracellular esterification sites. The formation of [3H]cholesteryl esters was not affected by cytoskeleton-disrupting drugs. A reduction of cellular pinocytosis by 75% did not reduce the formation of tracer-labelled esters, suggesting that membrane flow due to the energy-dependent pinocytosis is no major contributor to the intracellular transport of molecular cholesterol between plasma membranes and esterification sites. The formation of [3H]cholesteryl esters was not significantly affected by energy poisons (NaF and KCN) but was inhibited (to 50%) by chloroquine at 50 μM. This may indicate that membrane-derived cholesterol passes through the lysosomal compartment on its way to intracellular esterification sites.

Url:

https://api.istex.fr/ark:/67375/6H6-7184H25S-Q/fulltext.pdf

DOI: 10.1016/0005-2760(84)90258-3

Affiliations:

Finlande, Suède

Le document en format XML

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<term>Cyanides (pharmacology)</term>
<term>Cytochalasin B (pharmacology)</term>
<term>Fluorides (pharmacology)</term>
<term>Humans</term>
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<term>Cyanures (pharmacologie)</term>
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<term>Pinocytose ()</term>
<term>Poumon (métabolisme)</term>
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<term>Cellular pinocytosis</term>
<term>Chloroquine</term>
<term>Cholesterol exchange</term>
<term>Cholesterol exchange process</term>
<term>Cholesterol mass determination</term>
<term>Cholesteryl esters</term>
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<term>Cold saline</term>
<term>Conical centrifuge tubes</term>
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<term>Cultured mouse fibroblasts</term>
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<term>Energy poisons</term>
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<term>Ester formation</term>
<term>Esterification</term>
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<term>Exchangeable</term>
<term>Exchangeable cholesterol</term>
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<term>Lipid vesicles</term>
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<term>Membrane flow</term>
<term>Metabolic energy</term>
<term>Metabolic inhibitors</term>
<term>Model system</term>
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<term>Molecular exchange</term>
<term>Muscle cells</term>
<term>Pinocytosis</term>
<term>Plasma membranes</term>
<term>Results show</term>
<term>Surface transfer</term>
<term>Total cholesterol</term>
<term>Tracer</term>
<term>Tracer uptake</term>
<term>Unesterified cholesterol</term>
<term>Unpublished data</term>
<term>Unspecific binding</term>
<term>Vesicle</term>
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<term>Humains</term>
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<front><div type="abstract" xml:lang="en">Abstract: We have studied the intracellular fate of exchangeable cholesterol in a model system with lipid vesicles (cholesterol/phospholipid mole ratio 1:1) and cultured human lung fibroblasts. Exchangeable [3H]cholesterol in lipid vesicles was readily incorporated into cellular plasma membranes and transported to intracellular esterification sites. The formation of [3H]cholesteryl esters was not affected by cytoskeleton-disrupting drugs. A reduction of cellular pinocytosis by 75% did not reduce the formation of tracer-labelled esters, suggesting that membrane flow due to the energy-dependent pinocytosis is no major contributor to the intracellular transport of molecular cholesterol between plasma membranes and esterification sites. The formation of [3H]cholesteryl esters was not significantly affected by energy poisons (NaF and KCN) but was inhibited (to 50%) by chloroquine at 50 μM. This may indicate that membrane-derived cholesterol passes through the lysosomal compartment on its way to intracellular esterification sites.</div>
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Intracellular transport and esterification of exchangeable cholesterol in cultured human lung fibroblasts

Intracellular transport and esterification of exchangeable cholesterol in cultured human lung fibroblasts

Source :

Descripteurs français

English descriptors

Abstract

Links toward previous steps (curation, corpus...)

Le document en format XML

Pour manipuler ce document sous Unix (Dilib)

Pour mettre un lien sur cette page dans le réseau Wicri

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